Structural Characterization and Anticancer Activity of a New Anthraquinone from Senna velutina (Fabaceae)

In this study, a novel compound was isolated, identified, and its chemical structure determined from the extract of roots Senna velutina. addition, we sought to evaluate anticancer potential molecule against melanoma leukemic cell lines identify pathways death involved. To end, anthraquinone isolated barks S. velutina, analyzed by HPLC-DAD, molecular nuclear magnetic resonance (NMR). Subsequently, their cytotoxic activity evaluated (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method non-cancerous, melanoma, cells. The migration cells scratch assay. apoptosis process, caspase-3 activation, analysis mitochondrial membrane potential, measurement ROS were flow cytometry technique. pharmacological inhibitors NEC-1, RIP-1, BAPTA, Z-VAD, Z-DEVD used confirm related mechanisms. With results, it possible elucidate characterized as 2′-OH-Torosaol I. normal cells, showed no cytotoxicity in PBMC but reduced viability all evaluated. I inhibited chemotaxis B16F10-Nex2, SK-Mel-19, SK-Mel-28 SK-Mel-103. induced via intrinsic pathway with increased levels reactive oxygen species, activation caspase-3. demonstrated involvement necroptosis Ca2+ process confirmed caspase-dependent one main programmed Taken together, data characterize I, demonstrating application cancer therapy.